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Title: | SUSCEPTIBILIDAD ANTIMICROBIANA DE CEPAS DE Escherichia coli AISLADAS DE ALIMENTOS Y AGUAS RESIDUALES EN CUMANÁ, VENEZUELA |
Authors: | Martínez, Rosa E. Villalobos, Luz B. |
Keywords: | E. coli antibióticos alimentos aguas residuales susceptibility food wastewater |
Issue Date: | Aug-2008 |
Publisher: | Universidad de Oriente |
Abstract: | RESUMEN: La susceptibilidad a antimicrobianos de importancia clínica fue determinada para cepas de E. coli, 30 aisladas de alimentos y 30 de aguas servidas. La identifi cación se realizó siguiendo la bioquímica convencional y empleando galerías API ID 32 (BioMerieux). Las cepas aisladas fueron sometidas a ensayos de susceptibilidad mediante la técnica estandarizada por Bauer et al., (1966) con los antibióticos: cloranfenicol (30 μg), Ampicilina (10 μg), cefotaxime (30 μg), ceftazidime (30 μg), piperacilina (100 μg), gentamicina (10 μg), tobramicina (10 μg), ácido nalidixico (30 μg), levofl oxacina (5 μg), ciprofl oxacina (5 μg), trimetroprim-sulfametoxazol (23,75 μg+25 μg), y tetraciclina (30 μg). Los resultados de cada antibiótico fueron interpretados según normativas NCCLS, (2005). El 100% y 80 % de las cepas de E. coli aisladas de alimentos y aguas de desecho respectivamente mostraron resistencia a los antibióticos en prueba. En ambos grupos la más alta resistencia se observó frente ampicilina. ABSTRACT Susceptibility to clinically important antimicrobials was determined for 30 E. coli strains isolated from food and 30 from wastewater. The identifi cation was carried out following conventional biochemical tests and API 32 E (BioMerieux). Isolated strains underwent antimicrobial susceptibility testing was performed according to Bauer et al., (1966) disc diffusion method, with the following antibiotics: chloramphenicol (30 μg), ampicillin (10 μg), cefotaxime (30 μg), ceftazidime (30 μg), piperacillin (100 μg), gentamicin (10 μg), tobramycin (10 μg), nalidixic acid (30 μg), levofl oxacine (5 μg), ciprofl oxacine (5 μg), sulphamethoxazole-trimethoprim (23,75 μg+25 μg), and tetracycline (30 μg). Interpretative criteria for each antimicrobial tested were those recommended by NCCLS, (2005). A 100% and an 80% of E. coli strains isolated from food and wastewater, respectively, showed resistance to tested antibiotics. In both strain groups the highest resistance was against ampicillin. |
URI: | http://ri2.bib.udo.edu.ve:8080/jspui/handle/123456789/3950 |
ISSN: | 1315-0162 |
Appears in Collections: | Revista SABER - Vol. 20 - Nros. 1, 2 y 3 del año 2008 |
Files in This Item:
File | Description | Size | Format | |
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06 EVALUACION DE LA SUSCEPTIBILIDAD.pdf | 126,29 kB | Adobe PDF | View/Open |
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